oxiselect thiobarbituric acid reactive substances (tbars) assay kit  (Cell Biolabs Inc)

Journal: Scientific Reports

Article Title: Field direction of static magnetic fields influences kidney fibrosis progression through MAPK signaling and cell cycle alteration

doi: 10.1038/s41598-025-09077-w

Figure Lengend Snippet: Inhibition of p38 protected tubular epithelial cells from inward SMF-induced tubular damage. a , b Western blot representative images ( a ) and quantification ( b ) for OGG1, HIF-1α periostin, α-SMA, and fibronectin. c mRNA levels of CDK4 and OGG1 were analyzed using real-time qPCR (n = 4 in each group). d Representative images of cell cycle analysis by PI and Ki-67 staining (n = 3–6 in each group). e Proportions of HK-2 cells in each phase of the cell cycle quantified as percentages. f Intracellular ROS levels were assessed under inward SMFs and iP38 treatment. g Increased ratios of apoptotic cells were observed with inward SMF exposure, which decreased following treatment with a p38 MAPK inhibitor. Early apoptosis, late apoptosis, necrosis, and combined cell proportions were assessed under inward SMF exposure and p38 MAPK inhibition. The experiments were independently replicated at least three times, and the data are presented as mean ± SEM. Statistical significance is indicated as * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: To evaluate intracellular oxidative stress, ROS levels were measured with the OxiSelect TM Intracellular ROS Assay Kit (cat. STA-342, Cell Biolabs, San Diego, CA, USA). hTECs cultured under inward SMFs and treated with iP38 (0.1 μM, 1 μM) for 3 days were incubated with 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) for 1 h at 37 °C.

Techniques: Inhibition, Western Blot, Cell Cycle Assay, Staining